Nourseothricin N-Acetyl Transferase: A Positive Selection Marker for Mammalian Cells
نویسندگان
چکیده
منابع مشابه
Nourseothricin N-Acetyl Transferase: A Positive Selection Marker for Mammalian Cells
Development of Nourseothricin N-acetyl transferase (NAT) as a selection marker for mammalian cells is described. Mammalian cells are acutely susceptible to Nourseothricin, similar to the widely used drug Puromycin, and NAT allows for quick and robust selection of transfected/transduced cells in the presence of Nourseothricin. NAT is compatible with other selection markers puromycin, hygromycin,...
متن کاملNourseothricin acetyltransferease: a positive selectable marker for Toxoplasma gondii.
Molecular analysis of parasite genomes will require new molecular genetic tools. The nat1 gene of Streptomyces noursei encodes nourseothricin acetyltransferase, conferring resistance to the aminoglycoside antibiotic nourseothricin. Electroporation of nat1 cassettes into RH or Prugniaud strains of Toxoplasma gondii allows for selection of stable nourseothricin-resistant clones.
متن کاملIs urinary N-acetyl-beta-D-glucosaminidase a marker of urological abnormality in children?
Abstract Background: Hydronephrosis is the most common congenital condition that is detected by prenatal ultrasonography. Moreover, the widespread use of prenatal ultrasonography results in an increased recognition of fetal hydronephrosis. Prenatal hydronephrosis is diagnosed at an incidence of 1:100 to 1:500 by ultrasonographic studies. The presence of hydronephrosis is not synonymou...
متن کاملConstruction of T-vector derived from pBluescript ΙΙ SK with a positive selection marker, a rapid system for cloning
A rapid DNA cloning system is a research interest of many scientists. TA cloning is one of the methods used for the cloning of PCR-amplified DNA molecules. The TA cloning method is a convenient and labor-saving replacement to traditional, restriction enzyme-mediated cloning strategies. A T-vector called pBlueskript ΙΙ SK-1 with the lethal gene ccdB was designed to construct a positive selection...
متن کاملComputational Analysis of N-acetyl transferase in Tribolium castaneum
N-acetyl transferase (NAT) is responsible to catalyze the transfer of acetyl groups to arylamines from acetyl-CoA. Aralkylamine Nacetyl transferase (AANAT), which belongs to GCN5-related N-acetyl transferase member, is a globular 23-kDa cytosolic protein that forms a reversible regulatory complex with 14-3-3 proteins, AANAT regulates the daily cycle of melatonin biosynthesis in mammals, making ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PLoS ONE
سال: 2013
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0068509